A Strategy for the Sequential Recovery of Biomacromolecules from Red Macroalgae Porphyra umbilicalis Kützing
Artikel i vetenskaplig tidskrift, 2018

A nondestructive, multicomponent fractionation strategy has been developed to extract proteins and polysaccharides from the red macroalgae Porphyra umbilicalis collected along the west coast of Sweden and cultivated indoors under controlled conditions. First, a protein-rich fraction was extracted in an ice-cold alkaline solution. The overall protein content in Porphyra umbilicalis was estimated to be 30.6% of the dry weight, and out of that, 15.0% could be recovered. Water-soluble polysaccharides were then extracted from the insoluble residual fraction using sequential alkaline and acidic treatments at 90 °C for 4 h. Spectroscopic and chromatographic analyses of the polysaccharide fractions show that high-molecular-weight carrageenans were obtained from the alkaline extraction and a galactose-rich pectin substance was obtained from the acidic extraction. The insoluble fraction remaining after all extraction steps was rich in cellulose. An elemental analysis of Porphyra umbilicalis via scanning electron microscopy with energy-dispersive X-ray spectrometry (SEM-EDS) showed the presence of C, O, Na, Ca, Mg, Al, Cl, and S. However, no heavy metals or other toxic elements, such as Pb, Hg, and As, were found.

Författare

Niklas Wahlström

Kungliga Tekniska Högskolan (KTH)

Hanna Harrysson

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Ingrid Undeland

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Ulrica Edlund

Kungliga Tekniska Högskolan (KTH)

Industrial & Engineering Chemistry Research

0888-5885 (ISSN) 1520-5045 (eISSN)

Vol. 57 1 42-53

Ämneskategorier

Analytisk kemi

Livsmedelsteknik

Polymerteknologi

DOI

10.1021/acs.iecr.7b03768