Reshaping the Energy Landscape Transforms the Mechanism and Binding Kinetics of DNA Threading Intercalation
Artikel i vetenskaplig tidskrift, 2018

Molecules that bind DNA via threading intercalation show high binding affinity as well as slow dissociation kinetics, properties ideal for the development of anticancer drugs. To this end, it is critical to identify the specific molecular characteristics of threading intercalators that result in optimal DNA interactions. Using single-molecule techniques, we quantify the binding of a small metal-organic ruthenium threading intercalator (δ,δ-B) and compare its binding characteristics to a similar molecule with significantly larger threading moieties (δ,δ-P). The binding affinities of the two molecules are the same, while comparison of the binding kinetics reveals significantly faster kinetics for δ,δ-B. However, the kinetics is still much slower than that observed for conventional intercalators. Comparison of the two threading intercalators shows that the binding affinity is modulated independently by the intercalating section and the binding kinetics is modulated by the threading moiety. In order to thread DNA, δ,δ-P requires a "lock mechanism", in which a large length increase of the DNA duplex is required for both association and dissociation. In contrast, measurements of the force-dependent binding kinetics show that δ,δ-B requires a large DNA length increase for association but no length increase for dissociation from DNA. This contrasts strongly with conventional intercalators, for which almost no DNA length change is required for association but a large DNA length change must occur for dissociation. This result illustrates the fundamentally different mechanism of threading intercalation compared with conventional intercalation and will pave the way for the rational design of therapeutic drugs based on DNA threading intercalation.

Författare

Andrew G. Clark

Northeastern University

M. Nabuan Naufer

Northeastern University

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Per Lincoln

Chalmers, Kemi och kemiteknik, Kemi och biokemi

I. Rouzina

Ohio State University

T. Paramanathan

Bridgewater State University

Mark C. Williams

Northeastern University

Biochemistry

0006-2960 (ISSN) 1520-4995 (eISSN)

Vol. 57 5 614-619

Ämneskategorier

Fysikalisk kemi

Biokemi och molekylärbiologi

Annan medicinsk grundvetenskap

DOI

10.1021/acs.biochem.7b01036