Pentacyclic adenine: A versatile and exceptionally bright fluorescent DNA base analogue
Artikel i vetenskaplig tidskrift, 2018

Emissive base analogs are powerful tools for probing nucleic acids at the molecular level. Herein we describe the development and thorough characterization of pentacyclic adenine (pA), a versatile base analog with exceptional fluorescence properties. When incorporated into DNA, pA pairs selectively with thymine without perturbing the B-form structure and is among the brightest nucleobase analogs reported so far. Together with the recently established base analog acceptor qA nitro , pA allows accurate distance and orientation determination via Förster resonance energy transfer (FRET) measurements. The high brightness at emission wavelengths above 400 nm also makes it suitable for fluorescence microscopy, as demonstrated by imaging of single liposomal constructs coated with cholesterol-anchored pA-dsDNA, using total internal reflection fluorescence microscopy. Finally, pA is also highly promising for two-photon excitation at 780 nm, with a brightness (5.3 GM) that is unprecedented for a base analog.

Författare

Mattias Bood

Göteborgs universitet

Anders Foller Füchtbauer

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Moa Sandberg Wranne

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Jong Jin Ro

Pohang University of Science and Technology

Sangamesh Sarangamath

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Afaf El-Sagheer

Suez University

Deborah Rupert

Chalmers, Fysik, Biologisk fysik

Rachel S. Fisher

University of Edinburgh

Steven W. Magennis

University of Glasgow

Anita C. Jones

University of Edinburgh

Fredrik Höök

Chalmers, Fysik, Biologisk fysik

T. Brown

University of Oxford

Byeang Hyean Kim

Pohang University of Science and Technology

Anders Dahlén

AstraZeneca AB

Marcus Wilhelmsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Morten Grötli

Göteborgs universitet

Chemical Science

2041-6520 (ISSN) 2041-6539 (eISSN)

Vol. 9 14 3494-3502

Ämneskategorier

Atom- och molekylfysik och optik

Annan fysik

Teoretisk kemi

DOI

10.1039/c7sc05448c

Mer information

Senast uppdaterat

2018-04-18