Effect of microbial transglutaminase and setting condition on gel properties of blend fish protein isolate recovered by alkaline solubilisation/isoelectric precipitation
Artikel i vetenskaplig tidskrift, 2018

The effect of microbial transglutaminase (M‐TGase) (0–0.6 units g−1 sample) and setting condition (25 °C/180 min, 30 °C/120 min, 35 °C/60 min and 40 °C/30 min) on gel properties of blend protein isolate of gutted kilka and silver carp was studied. The protein isolate provided a good substrate for M‐TGase activity so that a low amount of M‐TGase (0.2 unit g−1 sample) substantially improved textural properties and water holding capacity (WHC) of the gels. Breaking force of the gels was positively affected by M‐TGase up to 0.6 unit g−1 sample, but it negatively affected their WHC. Prior setting at 25–35 °C increased the breaking force of proteins compared to directly heated gel, resulting in maximum breaking force at 35 °C/60 min. However, the setting at 40 °C/30 min caused proteolysis, which was reflected in higher amounts of TCA‐soluble peptides and gel weakening. Denser microstructure and higher myosin heavy chain polymerisation observed in the gels which experienced the setting was well correlated with improvement in textural properties.

Fish protein isolate, blend surimi, microbial transglutaminase, pH-shift method, setting condition


Mehdi Abdollahi

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Masoud Rezaei

Ali Jafarpour

Ingrid Undeland

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

International Journal of Food Science and Technology

0950-5423 (ISSN) 1365-2621 (eISSN)


Hållbar utveckling

Innovation och entreprenörskap


Biokemi och molekylärbiologi




Livsvetenskaper och teknik




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