Copper distribution in breast cancer cells detected by time-of-flight secondary ion mass spectrometry with delayed extraction methodology
Artikel i vetenskaplig tidskrift, 2018

Copper (Cu) is an essential transition metal ion that acts as a cofactor in many key enzymes. Cu is also needed for several hallmarks of cancer, and many copper-binding proteins are upregulated in various cancers. However, Cu-dependent cellular mechanisms and molecular pathways involved in cancer progression are not known. Fundamental to a better understanding of such phenomena is the investigation of the Cu subcellular distribution in cancer cells. The authors here show that Time-of-Flight Secondary Ion Mass Spectrometry combined with delayed extraction can be successfully applied to probe Cu localization in fixed MDA-MB-231 breast cancer cells providing subcellular resolution. Interestingly, the authors find Cu to be accumulated at nuclear regions of the cancer cells. Published by the AVS.

Författare

Stephanie Blockhuys

Chalmers, Biologi och bioteknik, Kemisk biologi

Per Malmberg

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Pernilla Wittung Stafshede

Chalmers, Biologi och bioteknik, Kemisk biologi

Biointerphases

1559-4106 (ISSN) 1934-8630 (eISSN)

Vol. 13 6 06E412

Ämneskategorier

Cellbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Cancer och onkologi

DOI

10.1116/1.5053814

PubMed

30577697

Mer information

Senast uppdaterat

2019-01-24