Rewiring carbon metabolism in yeast for high level production of aromatic chemicals
Artikel i vetenskaplig tidskrift, 2019

The production of bioactive plant compounds using microbial hosts is considered a safe, cost-competitive and scalable approach to their production. However, microbial production of some compounds like aromatic amino acid (AAA)-derived chemicals, remains an outstanding metabolic engineering challenge. Here we present the construction of a Saccharomyces cerevisiae platform strain able to produce high levels of p-coumaric acid, an AAA-derived precursor for many commercially valuable chemicals. This is achieved through engineering the AAA biosynthesis pathway, introducing a phosphoketalose-based pathway to divert glycolytic flux towards erythrose 4-phosphate formation, and optimizing carbon distribution between glycolysis and the AAA biosynthesis pathway by replacing the promoters of several important genes at key nodes between these two pathways. This results in a maximum p-coumaric acid titer of 12.5 g L−1 and a maximum yield on glucose of 154.9 mg g−1.

Saccharomyces cerevisiae

Författare

Quanli Liu

Chalmers, Biologi och bioteknik, Systembiologi

Tao Yu

Chalmers, Biologi och bioteknik, Systembiologi

Xiaowei Li

Chalmers, Biologi och bioteknik, Systembiologi

Yu Chen

Chalmers, Biologi och bioteknik, Systembiologi

Kate Campbell

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Danmarks Tekniske Universitet (DTU)

Yun Chen

Chalmers, Biologi och bioteknik, Systembiologi

Nature Communications

2041-1723 (ISSN)

Vol. 10 1 4976

Ämneskategorier

Mikrobiologi

Växtbioteknologi

Biokatalys och enzymteknik

DOI

10.1038/s41467-019-12961-5

Mer information

Senast uppdaterat

2019-11-08