Rewiring carbon metabolism in yeast for high level production of aromatic chemicals
Journal article, 2019

The production of bioactive plant compounds using microbial hosts is considered a safe, cost-competitive and scalable approach to their production. However, microbial production of some compounds like aromatic amino acid (AAA)-derived chemicals, remains an outstanding metabolic engineering challenge. Here we present the construction of a Saccharomyces cerevisiae platform strain able to produce high levels of p-coumaric acid, an AAA-derived precursor for many commercially valuable chemicals. This is achieved through engineering the AAA biosynthesis pathway, introducing a phosphoketalose-based pathway to divert glycolytic flux towards erythrose 4-phosphate formation, and optimizing carbon distribution between glycolysis and the AAA biosynthesis pathway by replacing the promoters of several important genes at key nodes between these two pathways. This results in a maximum p-coumaric acid titer of 12.5 g L−1 and a maximum yield on glucose of 154.9 mg g−1.

Saccharomyces cerevisiae

Author

Quanli Liu

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Tao Yu

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Xiaowei Li

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Yu Chen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Kate Campbell

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Technical University of Denmark (DTU)

Yun Chen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Nature Communications

2041-1723 (ISSN)

Vol. 10 1 4976

Subject Categories

Microbiology

Plant Biotechnology

Biocatalysis and Enzyme Technology

DOI

10.1038/s41467-019-12961-5

More information

Latest update

11/8/2019