Metabolic Profiling and Compound-Class Identification Reveal Alterations in Serum Triglyceride Levels in Mice Immunized with Human Vaccine Adjuvant Alum
Artikel i vetenskaplig tidskrift, 2020
Alum has been widely used as an adjuvant for human vaccines; however, the impact of Alum on host metabolism remains largely unknown. Herein, we applied mass spectrometry (MS) (liquid chromatography-MS)-based metabolic and lipid profiling to monitor the effects of the Alum adjuvant on mouse serum at 6, 24, 72, and 168 h post-vaccination. We propose a new strategy termed subclass identification and annotation for metabolomics for class-wise identification of untargeted metabolomics data generated from high-resolution MS. Using this approach, we identified and validated the levels of several lipids in mouse serum that were significantly altered following Alum administration. These lipids showed a biphasic response even 168 h after vaccination. The majority of the lipids were triglycerides (TAGs), where TAGs with long-chain unsaturated fatty acids (FAs) decreased at 24 h and TAGs with short-chain FAs decreased at 168 h. To our knowledge, this is the first report on the impact of human vaccine adjuvant Alum on the host metabolome, which may provide new insights into the mechanism of action of Alum. ©
Alum adjuvant
human vaccine
metabolomics
mass spectrometry
Författare
Sakda Khoomrung
Mahidol University
Chalmers, Biologi och bioteknik, Systembiologi
Intawat Nookaew
Chalmers, Biologi och bioteknik, Systembiologi
University of Arkansas for Medical Sciences
Partho Sen
Chalmers, Biologi och bioteknik, Systembiologi
T. A. Olafsdottir
Göteborgs universitet
Josefine Persson
Göteborgs universitet
Thomas Moritz
Sveriges lantbruksuniversitet (SLU)
P. Andersen
Statens Serum Institut
A. M. Harandi
Göteborgs universitet
University of British Columbia (UBC)
Jens B Nielsen
Chalmers, Biologi och bioteknik, Systembiologi
Journal of Proteome Research
1535-3893 (ISSN) 1535-3907 (eISSN)
Vol. 19 1 269-278Ämneskategorier
Farmaceutisk vetenskap
Farmakologi och toxikologi
Miljövetenskap
DOI
10.1021/acs.jproteome.9b00517
PubMed
31625748