Metabolic Profiling and Compound-Class Identification Reveal Alterations in Serum Triglyceride Levels in Mice Immunized with Human Vaccine Adjuvant Alum
Journal article, 2020
Alum has been widely used as an adjuvant for human vaccines; however, the impact of Alum on host metabolism remains largely unknown. Herein, we applied mass spectrometry (MS) (liquid chromatography-MS)-based metabolic and lipid profiling to monitor the effects of the Alum adjuvant on mouse serum at 6, 24, 72, and 168 h post-vaccination. We propose a new strategy termed subclass identification and annotation for metabolomics for class-wise identification of untargeted metabolomics data generated from high-resolution MS. Using this approach, we identified and validated the levels of several lipids in mouse serum that were significantly altered following Alum administration. These lipids showed a biphasic response even 168 h after vaccination. The majority of the lipids were triglycerides (TAGs), where TAGs with long-chain unsaturated fatty acids (FAs) decreased at 24 h and TAGs with short-chain FAs decreased at 168 h. To our knowledge, this is the first report on the impact of human vaccine adjuvant Alum on the host metabolome, which may provide new insights into the mechanism of action of Alum. ©
Alum adjuvant
human vaccine
metabolomics
mass spectrometry
Author
Sakda Khoomrung
Mahidol University
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Intawat Nookaew
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
University of Arkansas for Medical Sciences
Partho Sen
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
T. A. Olafsdottir
University of Gothenburg
Josefine Persson
University of Gothenburg
Thomas Moritz
Swedish University of Agricultural Sciences (SLU)
P. Andersen
Statens Serum Institut
A. M. Harandi
University of Gothenburg
University of British Columbia (UBC)
Jens B Nielsen
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Journal of Proteome Research
1535-3893 (ISSN) 1535-3907 (eISSN)
Vol. 19 1 269-278Subject Categories
Pharmaceutical Sciences
Pharmacology and Toxicology
Environmental Sciences
DOI
10.1021/acs.jproteome.9b00517
PubMed
31625748