Metabolic Profiling and Compound-Class Identification Reveal Alterations in Serum Triglyceride Levels in Mice Immunized with Human Vaccine Adjuvant Alum
Journal article, 2019

Alum has been widely used as an adjuvant for human vaccines; however, the impact of Alum on host metabolism remains largely unknown. Herein, we applied mass spectrometry (MS) (liquid chromatography-MS)-based metabolic and lipid profiling to monitor the effects of the Alum adjuvant on mouse serum at 6, 24, 72, and 168 h post-vaccination. We propose a new strategy termed subclass identification and annotation for metabolomics for class-wise identification of untargeted metabolomics data generated from high-resolution MS. Using this approach, we identified and validated the levels of several lipids in mouse serum that were significantly altered following Alum administration. These lipids showed a biphasic response even 168 h after vaccination. The majority of the lipids were triglycerides (TAGs), where TAGs with long-chain unsaturated fatty acids (FAs) decreased at 24 h and TAGs with short-chain FAs decreased at 168 h. To our knowledge, this is the first report on the impact of human vaccine adjuvant Alum on the host metabolome, which may provide new insights into the mechanism of action of Alum. ©

mass spectrometry

metabolomics

Alum adjuvant

human vaccine

Author

Sakda Khoomrung

Mahidol University

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Intawat Nookaew

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

University of Arkansas for Medical Sciences

Partho Sen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

T. A. Olafsdottir

University of Gothenburg

Josefine Persson

University of Gothenburg

Thomas Moritz

Swedish University of Agricultural Sciences (SLU)

P. Andersen

Statens Serum Institut

A. M. Harandi

University of Gothenburg

University of British Columbia (UBC)

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Journal of Proteome Research

1535-3893 (ISSN) 1535-3907 (eISSN)

Vol. In press

Subject Categories

Pharmaceutical Sciences

Pharmacology and Toxicology

Environmental Sciences

DOI

10.1021/acs.jproteome.9b00517

PubMed

31625748

More information

Latest update

11/19/2019