Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose
Artikel i vetenskaplig tidskrift, 2019

Background: Aromatic amino acids and their derivatives are valuable chemicals and are precursors for different industrially compounds. p-Coumaric acid is the main building block for complex secondary metabolites in commercial demand, such as flavonoids and polyphenols. Industrial scale production of this compound from yeast however remains challenging. Results: Using metabolic engineering and a systems biology approach, we developed a Saccharomyces cerevisiae platform strain able to produce 242 mg/L of p-coumaric acid from xylose. The same strain produced only 5.35 mg/L when cultivated with glucose as carbon source. To characterise this platform strain further, transcriptomic analysis was performed, comparing this strain's growth on xylose and glucose, revealing a strong up-regulation of the glyoxylate pathway alongside increased cell wall biosynthesis and unexpectedly a decrease in aromatic amino acid gene expression when xylose was used as carbon source. Conclusions: The resulting S. cerevisiae strain represents a promising platform host for future production of p-coumaric using xylose as a carbon source.

Xylose

p-Coumaric acid

RNAseq

Transcriptome

Saccharomyces cerevisiae

Författare

Gheorghe M. Borja

Danmarks Tekniske Universitet (DTU)

A. Rodriguez

Danmarks Tekniske Universitet (DTU)

Köpenhamns universitet

Kate Campbell

Chalmers, Biologi och bioteknik, Systembiologi

I. Borodina

Danmarks Tekniske Universitet (DTU)

Yun Chen

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Köpenhamns universitet

Danmarks Tekniske Universitet (DTU)

BioInnovation Institute

Microbial Cell Factories

1475-2859 (ISSN)

Vol. 18 1 191

Ämneskategorier

Bioprocessteknik

Mikrobiologi

Annan industriell bioteknik

DOI

10.1186/s12934-019-1244-4

Mer information

Senast uppdaterat

2019-11-19