Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose
Journal article, 2019

Background: Aromatic amino acids and their derivatives are valuable chemicals and are precursors for different industrially compounds. p-Coumaric acid is the main building block for complex secondary metabolites in commercial demand, such as flavonoids and polyphenols. Industrial scale production of this compound from yeast however remains challenging. Results: Using metabolic engineering and a systems biology approach, we developed a Saccharomyces cerevisiae platform strain able to produce 242 mg/L of p-coumaric acid from xylose. The same strain produced only 5.35 mg/L when cultivated with glucose as carbon source. To characterise this platform strain further, transcriptomic analysis was performed, comparing this strain's growth on xylose and glucose, revealing a strong up-regulation of the glyoxylate pathway alongside increased cell wall biosynthesis and unexpectedly a decrease in aromatic amino acid gene expression when xylose was used as carbon source. Conclusions: The resulting S. cerevisiae strain represents a promising platform host for future production of p-coumaric using xylose as a carbon source.

Xylose

p-Coumaric acid

RNAseq

Transcriptome

Saccharomyces cerevisiae

Author

Gheorghe M. Borja

Technical University of Denmark (DTU)

A. Rodriguez

Technical University of Denmark (DTU)

University of Copenhagen

Kate Campbell

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

I. Borodina

Technical University of Denmark (DTU)

Yun Chen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

University of Copenhagen

Technical University of Denmark (DTU)

BioInnovation Institute

Microbial Cell Factories

1475-2859 (ISSN)

Vol. 18 1 191

Subject Categories

Bioprocess Technology

Microbiology

Other Industrial Biotechnology

DOI

10.1186/s12934-019-1244-4

More information

Latest update

11/19/2019