Adsorption of Fibrinogen on Silica Surfaces-The Effect of Attached Nanoparticles
Artikel i vetenskaplig tidskrift, 2020

When a biomaterial is inserted into the body, proteins rapidly adsorb onto its surface, creating a conditioning protein film that functions as a link between the implant and adhering cells. Depending on the nano-roughness of the surface, proteins will adsorb in different amounts, with different conformations and orientations, possibly affecting the subsequent attachment of cells to the surface. Thus, modifications of the surface nanotopography of an implant may prevent biomaterial-associated infections. Fibrinogen is of particular importance since it contains adhesion epitopes that are recognized by both eukaryotic and prokaryotic cells, and can therefore influence the adhesion of bacteria. The aim of this study was to model adsorption of fibrinogen to smooth or nanostructured silica surfaces in an attempt to further understand how surface nanotopography may affect the orientation of the adsorbed fibrinogen molecule. We used a coarse-grained model, where the main body of fibrinogen (visible in the crystal structure) was modeled as rigid and the flexible α C-chains (not visible in the crystal structure) were modeled as completely disordered. We found that the elongated fibrinogen molecule preferably adsorbs in such a way that it protrudes further into solution on a nanostructured surface compared to a flat one. This implicates that the orientation on the flat surface increases its bio-availability.

nanotopography

coarse-grained modeling

nanoparticles

protein adsorption

fibrinogen

Författare

Kristin Hyltegren

Lunds universitet

Mats Hulander

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Martin Andersson

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Martin Andersson Group

M. Skepo

Lund Institute of Advanced Neutron and X-ray Science

Lunds universitet

Biomolecules

2218-273X (eISSN)

Vol. 10 3 413

Ämneskategorier

Fysikalisk kemi

Bioteknisk apparatteknik

Medicinska material och protesteknik

DOI

10.3390/biom10030413

PubMed

32155964

Mer information

Senast uppdaterat

2020-04-17