Single-vesicle imaging reveals lipid-selective and stepwise membrane disruption by monomeric α-synuclein
Artikel i vetenskaplig tidskrift, 2020

Neurodegenerative diseases are increasing among the world's population, but there are no cures. These disorders all involve proteins that assemble into amyloid fibers which results in brain cell death. Evidence suggests that association of these proteins with lipid membranes is crucial for both functional and pathological roles. In Parkinson's disease, the involved protein, α-synuclein, is thought to function in trafficking of lipid vesicles in the brain. In search of mechanistic origins, increasing focus is put on identifying neurotoxic reactions induced by membrane interactions. To contribute new clues to this question, we here employed a new surface-sensitive scattering microscopy technique. With this approach, we discovered that α-synuclein perturbs vesicles in a stepwise and lipid-dependent fashion already at very low protein coverage.

membrane interaction

α-synuclein

lipid vesicle

single-vesicle scattering

Författare

Jonas Hannestad

Chalmers, Fysik, Biologisk fysik

Sandra Rocha

Chalmers, Biologi och bioteknik, Kemisk biologi

Björn Agnarsson

Nano- och biofysik

Vladimir Zhdanov

Chalmers, Fysik, Biologisk fysik

Pernilla Wittung Stafshede

Chalmers, Biologi och bioteknik, Kemisk biologi

Fredrik Höök

Nano- och biofysik

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 117 25 14178-14186

Ämneskategorier

Biofysik

DOI

10.1073/pnas.1914670117

Mer information

Skapat

2020-06-29