Building blocks are synthesized on demand during the yeast cell cycle
Artikel i vetenskaplig tidskrift, 2020

For cells to replicate, a sufficient supply of biosynthetic precursors is needed, necessitating the concerted action of metabolism and protein synthesis during progressive phases of cell division. A global understanding of which biosynthetic processes are involved and how they are temporally regulated during replication is, however, currently lacking. Here, quantitative multiomics analysis is used to generate a holistic view of the eukaryal cell cycle, using the budding yeast Saccharomyces cerevisiae. Protein synthesis and central carbon pathways such as glycolysis and amino acid metabolism are shown to synchronize their respective abundance profiles with division, with pathway-specific changes in metabolite abundance also being reflected by a relative increase in mitochondrial volume, as shown by quantitative fluorescence microscopy. These results show biosynthetic precursor production to be temporally regulated to meet phase-specific demands of eukaryal cell division.


cell cycle


Saccharomyces cerevisiae

absolute quantitation


Kate Campbell

Chalmers, Biologi och bioteknik, Systembiologi

Jakub Westholm

Science for Life Laboratory (SciLifeLab)

Sergo Kasvandik

Tartu Ülikool

Francesca Di Bartolomeo

SINTEF industri

Chalmers, Biologi och bioteknik, Systembiologi

Maurizio Mormino

Chalmers, Biologi och bioteknik, Industriell bioteknik

Jens B Nielsen

BioInnovation Institute

Chalmers, Biologi och bioteknik, Systembiologi

Danmarks Tekniske Universitet (DTU)

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 117 14 7575-7583


Biokemi och molekylärbiologi





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