Building blocks are synthesized on demand during the yeast cell cycle
Journal article, 2020
For cells to replicate, a sufficient supply of biosynthetic precursors is needed, necessitating the concerted action of metabolism and protein synthesis during progressive phases of cell division. A global understanding of which biosynthetic processes are involved and how they are temporally regulated during replication is, however, currently lacking. Here, quantitative multiomics analysis is used to generate a holistic view of the eukaryal cell cycle, using the budding yeast Saccharomyces cerevisiae. Protein synthesis and central carbon pathways such as glycolysis and amino acid metabolism are shown to synchronize their respective abundance profiles with division, with pathway-specific changes in metabolite abundance also being reflected by a relative increase in mitochondrial volume, as shown by quantitative fluorescence microscopy. These results show biosynthetic precursor production to be temporally regulated to meet phase-specific demands of eukaryal cell division.
absolute quantitation
metabolism
cell cycle
Saccharomyces cerevisiae
multiomics
Author
Kate Campbell
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Jakub Westholm
Science for Life Laboratory (SciLifeLab)
Sergo Kasvandik
University of Tartu
Francesca Di Bartolomeo
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
SINTEF Industry
Maurizio Mormino
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Jens B Nielsen
Technical University of Denmark (DTU)
BioInnovation Institute
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Proceedings of the National Academy of Sciences of the United States of America
0027-8424 (ISSN) 1091-6490 (eISSN)
Vol. 117 14 7575-7583Subject Categories
Biochemistry and Molecular Biology
DOI
10.1073/pnas.1919535117
PubMed
32213592