Interpolymer complexation of a polymer brush
Licentiatavhandling, 2020

Controllable macromolecular gating between nanoscopic compartments is of high interest for single molecule studies of biological macromolecules. By definition, a good macromolecular gate should completely stop biomolecules, such as proteins, from crossing between compartments in its closed state while letting them pass in its open state. Polymer brushes of poly(ethylene glycol) have been proven excellent barriers for proteins in previous work, but are limited in terms of stimuli-responsive behaviour needed for macromolecular gating. In this thesis work, the pH reversible interpolymer complexation between a poly(ethylene glycol) brush and poly(methacrylic acid) was investigated as a potential macromolecular gating mechanism. Conclusions were based on the evaluation of the resulting surface complex using three surface sensitive characterisation techniques. Upon complexing at low pH, the polymer layer was found to adopt a shrunken state with significant behavioural changes, while completely reversing back to its initial state after a neutral pH had been introduced. This pH reversible interaction show great promise as a pH controlled macromolecular gating mechanism and calls for further studies with suitable nanostructures. To this end, the fabrication and properties of a new solid-state nanopore device is also presented, together with the direction needed for future work towards a new macromolecular gating system.

functional materials

pH responsive

macromolecular gating

polymer brush

Interpolymer complex

nanopores

Pater Noster room 2034 at floor 2 in forskarhus 1

Författare

John Andersson

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Andreas Dahlin Group

Optical properties of plasmonic nanopore arrays prepared by electron beam and colloidal lithography

Nanoscale Advances,; Vol. 1(2019)p. 4282-4289

Artikel i vetenskaplig tidskrift

Andersson, J, del Castillo G.F.D, Bilotto, P, Höök, F, and Dahlin, A Control of polymer brush morphology, rheology and protein repulsion by hydrogen bond complexation

SIMONANO (Single Molecule Analysis in Nanoscale Reaction)

Familjen Erling-Perssons stiftelse, 2018-02-01 -- 2019-12-31.

Styrkeområden

Nanovetenskap och nanoteknik (SO 2010-2017, EI 2018-)

Ämneskategorier

Biokemi och molekylärbiologi

Polymerteknologi

Materialkemi

Nanoteknik

Infrastruktur

Chalmers materialanalyslaboratorium

Nanotekniklaboratoriet

Licentiatuppsatser vid Institutionen för kemi och kemiteknik, Chalmers tekniska högskola: 2020:18

Utgivare

Chalmers tekniska högskola

Pater Noster room 2034 at floor 2 in forskarhus 1

Online

Mer information

Senast uppdaterat

2021-01-14