High-resolution macromolecular crystallography at the FemtoMAX beamline with time-over-threshold photon detection
Artikel i vetenskaplig tidskrift, 2021
Protein dynamics contribute to protein function on different time scales. Ultrafast X-ray diffraction snapshots can visualize the location and amplitude of atom displacements after perturbation. Since amplitudes of ultrafast motions are small, high-quality X-ray diffraction data is necessary for detection. Diffraction from bovine trypsin crystals using single femtosecond X-ray pulses was recorded at FemtoMAX, which is a versatile beamline of the MAX IV synchrotron. The time-over-threshold detection made it possible that single photons are distinguishable even under short-pulse low-repetition-rate conditions. The diffraction data quality from FemtoMAX beamline enables atomic resolution investigation of protein structures. This evaluation is based on the shape of the Wilson plot, cumulative intensity distribution compared with theoretical distribution, I/σ, Rmerge /Rmeas and CC1/2 statistics versus resolution. The FemtoMAX beamline provides an interesting alternative to X-ray free-electron lasers when studying reversible processes in protein crystals.
femtosecond
multilayer monochromator
macromolecular crystallography.
time-over-threshold
Författare
Maja Jensen
Göteborgs universitet
Viktor Ahlberg Gagnér
Göteborgs universitet
Juan Cabello Sánchez
Chalmers, Mikroteknologi och nanovetenskap (MC2), Terahertz- och millimetervågsteknik
Åsa U. J. Bengtsson
Lunds universitet
J.Carl Ekström
Max IV-laboratoriet
Tinna Björg Úlfarsdóttir
Göteborgs universitet
Maria-Jose Garcia-Bonete
Göteborgs universitet
Andrius Jurgilaitis
Max IV-laboratoriet
David Kroon
Max IV-laboratoriet
Van-Thai Pham
Max IV-laboratoriet
Vietnam Academy of Science and Technology
Stefano Checcia
Max IV-laboratoriet
Hélène Coudert-Alteirac
Max IV-laboratoriet
Siawosch Schewa
Technische Hochschule Lübeck
Manfred Rössle
Technische Hochschule Lübeck
Helena Rodilla
Chalmers, Mikroteknologi och nanovetenskap (MC2), Terahertz- och millimetervågsteknik
Jan Stake
Chalmers, Mikroteknologi och nanovetenskap (MC2), Terahertz- och millimetervågsteknik
Vitali Zhaunerchyk
Göteborgs universitet, Institutionen för fysik
Jörgen Larsson
Lunds universitet
Gergely Katona
Göteborgs universitet
Journal of Synchrotron Radiation
0909-0495 (ISSN) 1600-5775 (eISSN)
Vol. 28Ämneskategorier
Acceleratorfysik och instrumentering
Atom- och molekylfysik och optik
Strukturbiologi
DOI
10.1107/S1600577520014599