High-resolution macromolecular crystallography at the FemtoMAX beamline with time-over-threshold photon detection
Artikel i vetenskaplig tidskrift, 2021
Protein dynamics contribute to protein function on different time scales. Ultrafast X-ray diffraction snapshots can visualize the location and amplitude of atom displacements after perturbation. Since amplitudes of ultrafast motions are small, high-quality X-ray diffraction data is necessary for detection. Diffraction from bovine trypsin crystals using single femtosecond X-ray pulses was recorded at FemtoMAX, which is a versatile beamline of the MAX IV synchrotron. The time-over-threshold detection made it possible that single photons are distinguishable even under short-pulse low-repetition-rate conditions. The diffraction data quality from FemtoMAX beamline enables atomic resolution investigation of protein structures. This evaluation is based on the shape of the Wilson plot, cumulative intensity distribution compared with theoretical distribution, I/σ, Rmerge /Rmeas and CC1/2 statistics versus resolution. The FemtoMAX beamline provides an interesting alternative to X-ray free-electron lasers when studying reversible processes in protein crystals.
femtosecond
time-over-threshold
multilayer monochromator
macromolecular crystallography.
Författare
Maja Jensen
Göteborgs universitet
Viktor Ahlberg Gagnér
Göteborgs universitet
Juan Cabello Sánchez
Chalmers, Mikroteknologi och nanovetenskap, Terahertz- och millimetervågsteknik
Åsa U. J. Bengtsson
Lunds universitet
J.Carl Ekström
Max IV-laboratoriet
Tinna Björg Úlfarsdóttir
Göteborgs universitet
Maria-Jose Garcia-Bonete
Göteborgs universitet
Andrius Jurgilaitis
Max IV-laboratoriet
David Kroon
Max IV-laboratoriet
Van-Thai Pham
Vietnam Academy of Science and Technology
Max IV-laboratoriet
Stefano Checcia
Max IV-laboratoriet
Hélène Coudert-Alteirac
Max IV-laboratoriet
Siawosch Schewa
Technische Hochschule Lübeck
Manfred Rössle
Technische Hochschule Lübeck
Helena Rodilla
Chalmers, Mikroteknologi och nanovetenskap, Terahertz- och millimetervågsteknik
Jan Stake
Chalmers, Mikroteknologi och nanovetenskap, Terahertz- och millimetervågsteknik
Vitali Zhaunerchyk
Göteborgs universitet
Jörgen Larsson
Lunds universitet
Gergely Katona
Göteborgs universitet
Journal of Synchrotron Radiation
0909-0495 (ISSN) 1600-5775 (eISSN)
Vol. 28 64-70Ämneskategorier (SSIF 2011)
Acceleratorfysik och instrumentering
Atom- och molekylfysik och optik
Strukturbiologi
Infrastruktur
Kollberglaboratoriet
Fundament
Grundläggande vetenskaper
DOI
10.1107/S1600577520014599
PubMed
33399553
Relaterade dataset
X-ray diffraction images of bovine trypsin crystals recorded at the FemtoMAX beamline of Max IV synchrotron facility [dataset]
DOI: 10.5281/zenodo.4290177