Adaptation of a Microfluidic qPCR System for Enzyme Kinetic Studies
Artikel i vetenskaplig tidskrift, 2021

Microfluidic platforms offer a drastic increase in throughput while minimizing sample usage and hands-on time, which make them important tools for large-scale biological studies. A range of such systems have been developed for enzyme activity studies, although their complexity largely hinders their application to the wider scientific community. Here, we present adaptation of an easy-to-use commercial microfluidic qPCR system for performing enzyme kinetic studies. We demonstrate the functionality of the Fluidigm Biomark HD system (the Fluidigm system) by determining the kinetic properties of three oxidases in a resorufin-based fluorescence assay. The results obtained in the microfluidic system proved reproducible and comparable to the ones obtained in a standard microplate-based assay. With a wide range of easy-to-use, off-the-shelf components, the microfluidic system presents itself as a simple and customizable platform for high-throughput enzyme activity studies.

Författare

Elzbieta Rembeza

Chalmers, Biologi och bioteknik, Systembiologi

Martin Engqvist

Chalmers, Biologi och bioteknik, Systembiologi

ACS Omega

24701343 (eISSN)

Vol. 6 3 1985-1990

Ämneskategorier

Inbäddad systemteknik

Datavetenskap (datalogi)

Datorsystem

DOI

10.1021/acsomega.0c04918

PubMed

33521438

Mer information

Senast uppdaterat

2021-03-17