Inhibition of lpmos by fermented persimmon juice
Artikel i vetenskaplig tidskrift, 2021

Fermented persimmon juice, Kakishibu, has traditionally been used for wood and paper protection. This protective effect stems at least partially from inhibition of microbial cellulose degrading enzymes. The inhibitory effect of Kakishibu on lytic polysaccharide monooxygenases (LPMOs) and on a cocktail of cellulose hydrolases was studied, using three different cellulosic substrates. Dose dependent inhibition of LPMO activity by a commercial Kakishibu product was assessed for the well-characterized LPMO from Thermoascus aurantiacus TaAA9A, and the inhibitory effect was confirmed on five additional microbial LPMOs. The model tannin compound, tannic acid exhibited a similar inhibitory effect on TaAA9A as Kakishibu. It was further shown that both polyethylene glycol and tannase can alleviate the inhibitory effect of Kakishibu and tannic acid, indicating a likely mechanism of inhibition caused by unspecific tannin–protein interactions.

Cellulase

Inhibition

Tannins

LPMO

PEG

Kakishibu

Författare

Radina Tokin

Köpenhamns universitet

Johan Ipsen

Köpenhamns universitet

Mahesha M. Poojary

Köpenhamns universitet

P. E. Jensen

Köpenhamns universitet

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

Katja Salomon Johansen

Köpenhamns universitet

Biomolecules

2218-273X (eISSN)

Vol. 11 12 1890

Ämneskategorier

Polymerteknologi

Annan medicinsk grundvetenskap

Biokatalys och enzymteknik

DOI

10.3390/biom11121890

PubMed

34944533

Mer information

Senast uppdaterat

2021-12-29