Cinnamic acid and p-coumaric acid are metabolized to 4-hydroxybenzoic acid by Yarrowia lipolytica
Artikel i vetenskaplig tidskrift, 2023

Yarrowia lipolytica has been explored as a potential production host for flavonoid synthesis due to its high tolerance to aromatic acids and ability to supply malonyl-CoA. However, little is known about its ability to consume the precursors cinnamic and p-coumaric acid. In this study, we demonstrate that Y. lipolytica can consume these precursors through multiple pathways that are partially dependent on the cultivation medium. By monitoring the aromatic acid concentrations over time, we found that cinnamic acid is converted to p-coumaric acid. We identified potential proteins with a trans-cinnamate 4-monooxygenase activity in Y. lipolytica and constructed a collection of 15 knock-out strains to identify the genes responsible for the reaction. We identified YALI1_B28430g as the gene encoding for a protein that converts cinnamic acid to p-coumaric acid (designated as TCM1). By comparing different media compositions we found that complex media components (casamino acids and yeast extract) induce this pathway. Additionally, we discover the conversion of p-coumaric acid to 4-hydroxybenzoic acid. Our findings provide new insight into the metabolic capabilities of Y. lipolytica and hold great potential for the future development of improved strains for flavonoid production.

Yeast

4-hydroxybenzoate

Phenolic compounds

P450 protein

Organic acids degradation

Hydrolysate

Författare

Oliver Konzock

Chalmers, Life sciences, Systembiologi

Marta Tous Mohedano

Chalmers, Life sciences, Systembiologi

Irene Cibin

Yun Chen

Chalmers, Life sciences, Systembiologi

Joakim Norbeck

Utbildningsstöd

AMB Express

21910855 (eISSN)

Vol. 13 1 84

Systems metabolic engineering of yeast for production of flavonoids

Novo Nordisk Fonden (NNF18OC0034844), 2019-01-01 -- 2021-12-31.

Fett från trä: Optimering av jästsvampen Yarrowia lipolytica för produktion av matfett med definierad sammansättning från hydrolysat av lignocellulosa

Formas (2017-01281), 2018-01-01 -- 2021-12-31.

Ämneskategorier

Biokemi och molekylärbiologi

Mikrobiologi

DOI

10.1186/s13568-023-01590-3

PubMed

37561285

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Senast uppdaterat

2024-12-09