Performance and robustness analysis reveals phenotypic trade-offs in yeast
Artikel i vetenskaplig tidskrift, 2024

To design strains that can function efficiently in complex industrial settings, it is crucial to consider their robustness, that is, the stability of their performance when faced with perturbations. In the present study, we cultivated 24 Saccharomyces cerevisiae strains under conditions that simulated perturbations encountered during lignocellulosic bioethanol production, and assessed the performance and robustness of multiple phenotypes simultaneously. The observed negative correlations confirmed a trade-off between performance and robustness of ethanol yield, biomass yield, and cell dry weight. Conversely, the specific growth rate performance positively correlated with the robustness, presumably because of evolutionary selection for robust, fast-growing cells. The Ethanol Red strain exhibited both high performance and robustness, making it a good candidate for bioproduction in the tested perturbation space. Our results experimentally map the robustness-performance trade-offs, previously demonstrated mainly by single-phenotype and computational studies.

Författare

Cecilia Trivellin

Chalmers, Life sciences, Industriell bioteknik

Peter Rugbjerg

Enduro Genetics ApS

Chalmers, Life sciences, Industriell bioteknik

Lisbeth Olsson

Chalmers, Life sciences, Industriell bioteknik

Life Science Alliance

25751077 (eISSN)

Vol. 7 1

Microbial robustness - a key for sustainable and efficient biotechnology-based production

Novo Nordisk Fonden (NNF19OC00550444), 2019-09-01 -- 2024-08-31.

Ämneskategorier

Biokemi och molekylärbiologi

Mikrobiologi

Annan samhällsbyggnadsteknik

Styrkeområden

Energi

Hälsa och teknik

DOI

10.26508/lsa.202302215

PubMed

37903627

Mer information

Senast uppdaterat

2023-12-01