Comparison of CTX-M encoding plasmids present during the early phase of the ESBL pandemic in western Sweden
Artikel i vetenskaplig tidskrift, 2024

Plasmids encoding blaCTX-M genes have greatly shaped the evolution of E. coli producing extended-spectrum beta-lactamases (ESBL-E. coli) and adds to the global threat of multiresistant bacteria by promoting horizontal gene transfer (HGT). Here we screened the similarity of 47 blaCTX-M -encoding plasmids, from 45 epidemiologically unrelated and disperse ESBL-E. coli strains, isolated during the early phase (2009-2014) of the ESBL pandemic in western Sweden. Using optical DNA mapping (ODM), both similar and rare plasmids were identified. As many as 57% of the plasmids formed five ODM-plasmid groups of at least three similar plasmids per group. The most prevalent type (28%, IncIl, pMLST37) encoded blaCTX-M-15 (n = 10), blaCTX-M-3 (n = 2) or blaCTX-M-55 (n = 1). It was found in isolates of various sequence types (STs), including ST131. This could indicate ongoing local HGT as whole-genome sequencing only revealed similarities with a rarely reported, IncIl plasmid. The second most prevalent type (IncFII/FIA/FIB, F1:A2:B20) harboring blaCTX-M-27, was detected in ST131-C1-M27 isolates, and was similar to plasmids previously reported for this subclade. The results also highlight the need for local surveillance of plasmids and the importance of temporospatial epidemiological links so that detection of a prevalent plasmid is not overestimated as a potential plasmid transmission event in outbreak investigations.

Författare

Moa Wranne

Göteborgs universitet

Chalmers, Life sciences, Kemisk biologi

N. Karami

Sahlgrenska universitetssjukhuset

Göteborgs universitet

Sriram Kesarimangalam

Chalmers, Life sciences, Kemisk biologi

Daniel Jaén-Luchoro

Göteborgs universitet

Sahlgrenska universitetssjukhuset

Shora Yazdanshenas

Sahlgrenska universitetssjukhuset

Yii Lih Lin

Göteborgs universitet

Chalmers, Life sciences, Kemisk biologi

Arpitha Kabbinale

Carl-Fredrik Flach

Göteborgs universitet

Fredrik Westerlund

Göteborgs universitet

Chalmers, Life sciences, Kemisk biologi

Christina Åhrén

Göteborgs universitet

Swedish Strategic Program against Antimicrobial Resistance (Strama)

Scientific Reports

2045-2322 (ISSN) 20452322 (eISSN)

Vol. 14 1 11880- 11880

Genomic diagnostics beyond the sequence (BeyondSeq)

Europeiska kommissionen (EU) (EC/H2020/634890), 2015-06-01 -- 2019-12-31.

Ämneskategorier

Mikrobiologi

Mikrobiologi inom det medicinska området

DOI

10.1038/s41598-024-62663-2

PubMed

38789462

Mer information

Senast uppdaterat

2024-08-02