Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry
Artikel i vetenskaplig tidskrift, 2007

The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.

Författare

R. Kumar

University of Southampton

Afaf El-Sagheer

Chalmers

John Tumpane

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Per Lincoln

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Marcus Wilhelmsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

T. Brown

University of Southampton

Journal of the American Chemical Society

0002-7863 (ISSN) 1520-5126 (eISSN)

Vol. 129 21 6859-6864

Ämneskategorier

Kemi

DOI

10.1021/ja070273v