Mechanically manipulating the DNA threading intercalation rate
Artikel i vetenskaplig tidskrift, 2008

The dumbbell shaped binuclear ruthenium complex ΔΔ-P requires transiently melted DNA in order to thread through the DNA bases and intercalate DNA. Because such fluctuations are rare at room temperature, the binding rates are extremely low in bulk experiments. Here, single DNA molecule stretching is used to lower the barrier to DNA melting, resulting in direct mechanical manipulation of the barrier to DNA binding by the ligand. The rate of DNA threading depends exponentially on force, consistent with theoretical predictions. From the observed force dependence of the binding rate, we demonstrate that only one base pair must be transiently melted for DNA threading to occur. Copyright © 2008 American Chemical Society.


T. Paramanathan

Northeastern University

Fredrik Westerlund

Köpenhamns universitet

M. J. McCauley

Northeastern University

I. Rouzina

University of Minnesota

Per Lincoln

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

M. C. Williams

Northeastern University

Journal of the American Chemical Society

0002-7863 (ISSN) 1520-5126 (eISSN)

Vol. 130 12 3752-+





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