A novel ulvan lyase family with broad-spectrum activity from the ulvan utilisation loci of Formosa agariphila KMM 3901
Artikel i vetenskaplig tidskrift, 2018

Ulvan, which is one of the major structural polysaccharides of the cell walls of green macroalgae, is degraded by ulvan lyases via the β-elimination mechanism with the release of oligosaccharides that have unsaturated 4-deoxy-L-threo-hex-4-enopyranosiduronic acid (∆) at the non-reducing end. These ulvan lyases belong to the PL24 or PL25 or PL28 family in the CAZy database. In this study, we identify and biochemically characterise a periplasmic novel broad-spectrum ulvan lyase from Formosa agariphila KMM 3901. The lyase was overexpressed in Escherichia coli, and the purified recombinant enzyme depolymerised ulvan in an endolytic manner with a Kmof 0.77 mg/ml, and displayed optimum activity at 40 °C and pH 8. This lyase also degraded heparan sulphate and chondroitin sulphate. Detailed analyses of the end-products of the enzymatic degradation of ulvan using1H- and13C-NMR and LC-MS revealed an unsaturated disaccharide (∆Rha3S) and a tetrasaccharide (∆Rha3S-Xyl-Rha) as the principal end-products. In contrast to the previously described ulvan lyases, this novel lyase is mostly composed of α-helices that form an (α/α)6incomplete toroid domain and displays a remarkably broad-spectrum activity. This novel lyase is the first member of a new family of ulvan lyases.


Venkat Rao Konasani

Chalmers, Biologi och bioteknik, Industriell bioteknik

Chunsheng Jin

Göteborgs universitet

Niclas G. Karlsson

Göteborgs universitet

Eva Albers

Chalmers, Biologi och bioteknik, Industriell bioteknik

Scientific Reports

2045-2322 (ISSN) 20452322 (eISSN)

Vol. 8 1 14713

Produktionssystem för alger med högvärdiga tillämpningar (Sweaweed)

Stiftelsen för Strategisk forskning (SSF) (RBP14-0045), 2021-01-01 -- 2021-12-31.

Stiftelsen för Strategisk forskning (SSF) (RBP14-0045), 2015-01-01 -- 2019-12-31.


Biokemi och molekylärbiologi


Biokatalys och enzymteknik



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