Mig1 localization exhibits biphasic behavior which is controlled by both metabolic and regulatory roles of the sugar kinases
Artikel i vetenskaplig tidskrift, 2020

Glucose, fructose and mannose are the preferred carbon/energy sources for the yeastSaccharomyces cerevisiae. Absence of preferred energy sources activates glucose derepression, which is regulated by the kinase Snf1. Snf1 phosphorylates the transcriptional repressor Mig1, which results in its exit from the nucleus and subsequent derepression of genes. In contrast, Snf1 is inactive when preferred carbon sources are available, which leads to dephosphorylation of Mig1 and its translocation to the nucleus where Mig1 acts as a transcription repressor. Here we revisit the role of the three hexose kinases, Hxk1, Hxk2 and Glk1, in glucose de/repression. We demonstrate that all three sugar kinases initially affect Mig1 nuclear localization upon addition of glucose, fructose and mannose. This initial import of Mig1 into the nucleus was temporary; for continuous nucleocytoplasmic shuttling of Mig1, Hxk2 is required in the presence of glucose and mannose and in the presence of fructose Hxk2 or Hxk1 is required. Our data suggest that Mig1 import following exposure to preferred energy sources is controlled via two different pathways, where (1) the initial import is regulated by signals derived from metabolism and (2) continuous shuttling is regulated by the Hxk2 and Hxk1 proteins. Mig1 nucleocytoplasmic shuttling appears to be important for the maintenance of the repressed state in which Hxk1/2 seems to play an essential role.

Glucose repression

Localization

Hexose

Mig1

Hexokinase

Yeast

Oscillation

Microfluidic

Författare

Gregor W. Schmidt

Eidgenössische Technische Hochschule Zürich (ETH)

Niek Welkenhuysen

Chalmers, Biologi och bioteknik, Systembiologi

Göteborgs universitet

Tian Ye

Göteborgs universitet

Marija Cvijovic

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Göteborgs universitet

Stefan Hohmann

Chalmers, Biologi och bioteknik

Molecular Genetics and Genomics

1617-4615 (ISSN) 1617-4623 (eISSN)

Vol. 295 6 1489-1500

Ämneskategorier

Cellbiologi

Cell- och molekylärbiologi

Utvecklingsbiologi

DOI

10.1007/s00438-020-01715-4

PubMed

32948893

Mer information

Senast uppdaterat

2020-11-04