Phosphorylated CtIP bridges DNA to promote annealing of broken ends
Artikel i vetenskaplig tidskrift, 2020

The DNA of our cells is constantly exposed to various types of damaging agents. One of the most critical types of damage is when both strands of the DNA break, and thus such breaks need to be efficiently repaired. It is known that CtIP promotes nucleases in DNA break repair. Here we show that CtIP can also hold the two DNA strands together in solution when DNA is free to move, using novel methodology that allows the monitoring of thousands of single DNA molecules in nanofabricated devices. DNA bridging likely facilitates the enzymatic repair steps and identifies novel CtIP functions that are crucial for repairing broken DNA.

Författare

Robin Öz

Chalmers, Biologi och bioteknik, Kemisk biologi

Sean Howard

Universita della Svizzera italiana

Rajhans Sharma

Chalmers, Biologi och bioteknik, Kemisk biologi

Hanna Törnkvist

Student vid Chalmers

Ilaria Ceppi

Eidgenössische Technische Hochschule Zürich (ETH)

Universita della Svizzera italiana

Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Erik Kristiansson

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Petr Cejka

Eidgenössische Technische Hochschule Zürich (ETH)

Universita della Svizzera italiana

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 117 35 21403-21412

Styrkeområden

Nanovetenskap och nanoteknik

Ämneskategorier

Cell- och molekylärbiologi

Biologiska vetenskaper

DOI

10.1073/pnas.2008645117

PubMed

32817418

Mer information

Senast uppdaterat

2023-05-16