Improving biobutanol production in engineered Saccharomyces cerevisiae by manipulation of acetyl-CoA metabolism
Journal article, 2013

Recently, butanols (1-butanol, 2-butanol and iso-butanol) have generated attention as alternative gasoline additives. Butanols have several properties favorable in comparison to ethanol, and strong interest therefore exists in the reconstruction of the 1-butanol pathway in commonly used industrial microorganisms. In the present study, the biosynthetic pathway for 1-butanol production was reconstructed in the yeast Saccharomyces cerevisiae. In addition to introducing heterologous enzymes for butanol production, we engineered yeast to have increased flux toward cytosolic acetyl-CoA, the precursor metabolite for 1-butanol biosynthesis. This was done through introduction of a plasmid-containing genes for alcohol dehydrogenase (ADH2), acetaldehyde dehydrogenase (ALD6), acetyl-CoA synthetase (ACS), and acetyl-CoA acetyltransferase (ERG10), as well as the use of strains containing deletions in the malate synthase (MLS1) or citrate synthase (CIT2) genes. Our results show a trend to increased butanol production in strains engineered for increased cytosolic acetyl-CoA levels, with the best-producing strains having maximal butanol titers of 16.3 mg/l. This represents a 6.5-fold improvement in butanol titers compared to previous values reported for yeast and demonstrates the importance of an improved cytosolic acetyl-CoA supply for heterologous butanol production by this organism.

Metabolic engineering

Biofuel

Acetyl-coenzyme A

Saccharomyces cerevisiae

Synthetic biology

Biobutanol

Author

Anastasia Krivoruchko

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Cristina Serrano-Amatriain

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Yun Chen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Verena Siewers

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Journal of Industrial Microbiology and Biotechnology

1367-5435 (ISSN) 1476-5535 (eISSN)

Vol. 40 9 1051-1056

Subject Categories

Bioinformatics and Systems Biology

DOI

10.1007/s10295-013-1296-0

More information

Created

10/7/2017