Microbial acetyl-CoA metabolism and metabolic engineering
Journal article, 2015

Recent concerns over the sustainability of petrochemical-based processes for production of desired chemicals have fueled research into alternative modes of production. Metabolic engineering of microbial cell factories such as Saccharomyces cerevisiae and Escherichia coli offers a sustainable and flexible alternative for the production of various molecules. Acetyl-CoA is a key molecule in microbial central carbon metabolism and is involved in a variety of cellular processes. In addition, it functions as a precursor for many molecules of biotechnological relevance. Therefore, much interest exists in engineering the metabolism around the acetyl-CoA pools in cells in order to increase product titers. Here we provide an overview of the acetyl-CoA metabolism in eukaryotic and prokaryotic microbes (with a focus on S. cerevisiae and E. coli), with an emphasis on reactions involved in the production and consumption of acetyl-CoA. In addition, we review various strategies that have been used to increase acetyl-CoA production in these microbes.

Yeast

Central carbon metabolism

Bacteria

Industrial biotechnology

Acetyl-CoA

Author

Anastasia Krivoruchko

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Yiming Zhang

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Verena Siewers

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Yun Chen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Metabolic Engineering

1096-7176 (ISSN) 1096-7184 (eISSN)

Vol. 28 28-42

Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO)

European Commission (FP7), 2010-01-01 -- 2014-12-31.

Areas of Advance

Energy

Life Science Engineering (2010-2018)

Subject Categories

Microbiology

DOI

10.1016/j.ymben.2014.11.009

More information

Created

10/7/2017