Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules
Journal article, 2016
The rapid spread of antibiotic resistance - currently one of the greatest threats to human health according to WHO - is to a large extent enabled by plasmid-mediated horizontal transfer of resistance genes. Rapid identification and characterization of plasmids is thus important both for individual clinical outcomes and for epidemiological monitoring of antibiotic resistance. Toward this aim, we have developed an optical DNA mapping procedure where individual intact plasmids are elongated within nanofluidic channels and visualized through fluorescence microscopy, yielding barcodes that reflect the underlying sequence. The assay rapidly identifies plasmids through statistical comparisons with barcodes based on publicly available sequence repositories and also enables detection of structural variations. Since the assay yields holistic sequence information for individual intact plasmids, it is an ideal complement to next generation sequencing efforts which involve reassembly of sequence reads from fragmented DNA molecules. The assay should be applicable in microbiology labs around the world in applications ranging from fundamental plasmid biology to clinical epidemiology and diagnostics.
Author
Lena Nyberg
Chalmers, Biology and Biological Engineering, Chemical Biology
Mahmood Saair Quaderi
Lund University
Chalmers, Biology and Biological Engineering, Chemical Biology
Gustav Emilsson
Chalmers, Physics, Bionanophotonics
N. Karami
University of Gothenburg
E. Lagerstedt
Lund University
Vilhelm Müller
Chalmers, Biology and Biological Engineering, Chemical Biology
C. Noble
Lund University
S. Hammarberg
Lund University
A. Nilsson
Lund University
F. Sjoberg
University of Gothenburg
Joachim Fritzsche
Chalmers, Physics, Chemical Physics
L. Sandegren
Uppsala University
T. Ambjornsson
Lund University
Fredrik Westerlund
Chalmers, Biology and Biological Engineering, Chemical Biology
Erik Kristiansson
Chalmers, Mathematical Sciences, Applied Mathematics and Statistics
Scientific Reports
2045-2322 (ISSN) 20452322 (eISSN)
Vol. 6 30410Driving Forces
Sustainable development
Innovation and entrepreneurship
Areas of Advance
Nanoscience and Nanotechnology
Life Science Engineering (2010-2018)
Subject Categories
Biophysics
Microbiology
Microbiology in the medical area
DOI
10.1038/srep30410
PubMed
27460437