Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules
Artikel i vetenskaplig tidskrift, 2016

The rapid spread of antibiotic resistance - currently one of the greatest threats to human health according to WHO - is to a large extent enabled by plasmid-mediated horizontal transfer of resistance genes. Rapid identification and characterization of plasmids is thus important both for individual clinical outcomes and for epidemiological monitoring of antibiotic resistance. Toward this aim, we have developed an optical DNA mapping procedure where individual intact plasmids are elongated within nanofluidic channels and visualized through fluorescence microscopy, yielding barcodes that reflect the underlying sequence. The assay rapidly identifies plasmids through statistical comparisons with barcodes based on publicly available sequence repositories and also enables detection of structural variations. Since the assay yields holistic sequence information for individual intact plasmids, it is an ideal complement to next generation sequencing efforts which involve reassembly of sequence reads from fragmented DNA molecules. The assay should be applicable in microbiology labs around the world in applications ranging from fundamental plasmid biology to clinical epidemiology and diagnostics.

Författare

Lena Nyberg

Chalmers, Biologi och bioteknik, Kemisk biologi

Mahmood Saair Quaderi

Chalmers, Biologi och bioteknik, Kemisk biologi

Lunds universitet

Gustav Emilsson

Chalmers, Fysik, Bionanofotonik

N. Karami

Sahlgrenska akademin

E. Lagerstedt

Lunds universitet

Vilhelm Müller

Chalmers, Biologi och bioteknik, Kemisk biologi

C. Noble

Lunds universitet

S. Hammarberg

Lunds universitet

A. Nilsson

Lunds universitet

F. Sjoberg

Sahlgrenska akademin

Joachim Fritzsche

Chalmers, Fysik, Kemisk fysik

L. Sandegren

Uppsala universitet

T. Ambjornsson

Lunds universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Scientific Reports

2045-2322 (ISSN)

Vol. 6 30410

Drivkrafter

Hållbar utveckling

Innovation och entreprenörskap

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

DOI

10.1038/srep30410

PubMed

27460437

Mer information

Senast uppdaterat

2018-07-27