Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules
Artikel i vetenskaplig tidskrift, 2016

The rapid spread of antibiotic resistance - currently one of the greatest threats to human health according to WHO - is to a large extent enabled by plasmid-mediated horizontal transfer of resistance genes. Rapid identification and characterization of plasmids is thus important both for individual clinical outcomes and for epidemiological monitoring of antibiotic resistance. Toward this aim, we have developed an optical DNA mapping procedure where individual intact plasmids are elongated within nanofluidic channels and visualized through fluorescence microscopy, yielding barcodes that reflect the underlying sequence. The assay rapidly identifies plasmids through statistical comparisons with barcodes based on publicly available sequence repositories and also enables detection of structural variations. Since the assay yields holistic sequence information for individual intact plasmids, it is an ideal complement to next generation sequencing efforts which involve reassembly of sequence reads from fragmented DNA molecules. The assay should be applicable in microbiology labs around the world in applications ranging from fundamental plasmid biology to clinical epidemiology and diagnostics.


Lena Nyberg

Chalmers, Biologi och bioteknik, Kemisk biologi

Mahmood Saair Quaderi

Lunds universitet

Chalmers, Biologi och bioteknik, Kemisk biologi

Gustav Emilsson

Chalmers, Fysik, Bionanofotonik

N. Karami

Göteborgs universitet

E. Lagerstedt

Lunds universitet

Vilhelm Müller

Chalmers, Biologi och bioteknik, Kemisk biologi

C. Noble

Lunds universitet

S. Hammarberg

Lunds universitet

A. Nilsson

Lunds universitet

F. Sjoberg

Göteborgs universitet

Joachim Fritzsche

Chalmers, Fysik, Kemisk fysik

L. Sandegren

Uppsala universitet

T. Ambjornsson

Lunds universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Erik Kristiansson

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Scientific Reports

2045-2322 (ISSN) 20452322 (eISSN)

Vol. 6 30410


Hållbar utveckling

Innovation och entreprenörskap


Nanovetenskap och nanoteknik

Livsvetenskaper och teknik (2010-2018)




Mikrobiologi inom det medicinska området





Mer information

Senast uppdaterat