Detailed characterization of plasmids carrying resistance genes using optical DNA mapping
Paper in proceedings, 2016

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

Author

Vilhelm Müller

Chalmers, Biology and Biological Engineering, Chemical Biology

Lena Nyberg

Chalmers, Biology and Biological Engineering, Chemical Biology

Mahmood Saair Quaderi

Chalmers, Biology and Biological Engineering

T. Ambjornsson

Lund University

L. Sandegren

Uppsala University

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

1561-1562

Subject Categories

Nano Technology

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2/1/2018 8