Detailed characterization of plasmids carrying resistance genes using optical DNA mapping
Paper i proceeding, 2016

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

Författare

Vilhelm Müller

Chalmers, Biologi och bioteknik, Kemisk biologi

Lena Nyberg

Chalmers, Biologi och bioteknik, Kemisk biologi

Mahmood Saair Quaderi

Chalmers, Biologi och bioteknik

T. Ambjornsson

Lunds universitet

L. Sandegren

Uppsala universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

1561-1562

Ämneskategorier

Nanoteknik

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Senast uppdaterat

2018-02-01