An LC-QToF MS based method for untargeted metabolomics of human fecal samples
Journal article, 2020
Introduction: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. Objectives: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. Methods: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. Results: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. Conclusion: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.
Method optimization
Freeze-drying
XCMS
Untargeted fecal metabolomics
Short-chain fatty acids
Fecal sample
Author
Ken Cheng
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Carl Brunius
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Rikard Fristedt
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Rikard Landberg
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Metabolomics
1573-3882 (ISSN) 1573-3890 (eISSN)
Vol. 16 4 46Nya strategier för prevention av livsstilsrelaterade sjukdomar genom påverkan av kost och mikrobiota
Swedish Research Council (VR) (2017-05840), 2018-01-01 -- 2021-12-31.
Subject Categories
Biological Sciences
DOI
10.1007/s11306-020-01669-z
PubMed
32246267