An LC-QToF MS based method for untargeted metabolomics of human fecal samples
Journal article, 2020

Introduction: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. Objectives: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. Methods: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. Results: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. Conclusion: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.

Method optimization

Freeze-drying

XCMS

Untargeted fecal metabolomics

Short-chain fatty acids

Fecal sample

Author

Ken Cheng

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Carl Brunius

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Rikard Fristedt

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Rikard Landberg

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Metabolomics

1573-3882 (ISSN) 1573-3890 (eISSN)

Vol. 16 4 46

Nya strategier för prevention av livsstilsrelaterade sjukdomar genom påverkan av kost och mikrobiota

Swedish Research Council (VR), 2018-01-01 -- 2021-12-31.

Subject Categories

Biological Sciences

DOI

10.1007/s11306-020-01669-z

PubMed

32246267

More information

Latest update

6/5/2020 6