Building blocks are synthesized on demand during the yeast cell cycle
Journal article, 2020

For cells to replicate, a sufficient supply of biosynthetic precursors is needed, necessitating the concerted action of metabolism and protein synthesis during progressive phases of cell division. A global understanding of which biosynthetic processes are involved and how they are temporally regulated during replication is, however, currently lacking. Here, quantitative multiomics analysis is used to generate a holistic view of the eukaryal cell cycle, using the budding yeast Saccharomyces cerevisiae. Protein synthesis and central carbon pathways such as glycolysis and amino acid metabolism are shown to synchronize their respective abundance profiles with division, with pathway-specific changes in metabolite abundance also being reflected by a relative increase in mitochondrial volume, as shown by quantitative fluorescence microscopy. These results show biosynthetic precursor production to be temporally regulated to meet phase-specific demands of eukaryal cell division.

Saccharomyces cerevisiae

multiomics

cell cycle

absolute quantitation

metabolism

Author

Kate Campbell

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jakub Westholm

Science for Life Laboratory (SciLifeLab)

Sergo Kasvandik

University of Tartu

Francesca Di Bartolomeo

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Maurizio Mormino

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 117 14 7575-7583

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1073/pnas.1919535117

PubMed

32213592

More information

Latest update

7/3/2020 6