Mechanical characterization of base analogue modified nucleic acids by force spectroscopy
Journal article, 2021

We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study highlights the benefit of using base-modified nucleic acids in force-spectroscopy.

Author

Vinoth Sundar Rajan

Chalmers, Biology and Biological Engineering, Chemical Biology

Chalmers, Chemistry and Chemical Engineering

Xavier Viader-Godoy

University of Barcelona

Yii Lih Lin

Chalmers, Biology and Biological Engineering, Chemical Biology

Uttama Dutta

Chalmers, Chemistry and Chemical Engineering

Chalmers, Biology and Biological Engineering

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

Marcus Wilhelmsson

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Published in

Physical Chemistry Chemical Physics

1463-9076 (ISSN) 1463-9084 (eISSN)

Vol. 23 Issue 26 p. 14151-14155

Categorizing

Subject Categories (SSIF 2011)

Analytical Chemistry

Biomedical Laboratory Science/Technology

Biophysics

Identifiers

DOI

10.1039/d1cp01985f

PubMed

34180930

More information

Latest update

2/25/2025