An inverse association between plasma benzoxazinoid metabolites and PSA after rye intake in men with prostate cancer revealed with a new method
Journal article, 2022

Prostate cancer (PC) is a common cancer among men, and preventive strategies are warranted. Benzoxazinoids (BXs) in rye have shown potential against PC in vitro but human studies are lacking. The aim was to establish a quantitative method for analysis of BXs and investigate their plasma levels after a whole grain/bran rye vs refined wheat intervention, as well as exploring their association with PSA, in men with PC. A quantitative method for analysis of 22 BXs, including novel metabolites identified by mass spectrometry and NMR, was established, and applied to plasma samples from a randomized crossover study where patients with indolent PC (n = 17) consumed 485 g whole grain rye/rye bran or fiber supplemented refined wheat daily for 6 wk. Most BXs were significantly higher in plasma after rye (0.3–19.4 nmol/L in plasma) vs. refined wheat (0.05–2.9 nmol/L) intake. HBOA-glc, 2-HHPAA, HBOA-glcA, 2-HPAA-glcA were inversely correlated to PSA in plasma (p < 0.04). To conclude, BXs in plasma, including metabolites not previously analyzed, were quantified. BX metabolites were significantly higher after rye vs refined wheat consumption. Four BX-related metabolites were inversely associated with PSA, which merits further investigation.


Elise Nordin

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Stine K. Steffensen

Aarhus University

Bente B. Laursen

Aarhus University

Sven Olof Andersson

Örebro University

Jan Erik Johansson

Örebro University

Per Åman

Swedish University of Agricultural Sciences (SLU)

Göran Hallmans

Umeå University

Michael Borre

Arhus Universitetshospital

Dan Stærk

University of Copenhagen

Kati Hanhineva

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

University of Eastern Finland

Inge S. Fomsgaard

Aarhus University

Rikard Landberg

Umeå University

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Scientific Reports

2045-2322 (ISSN) 20452322 (eISSN)

Vol. 12 1 5260

Subject Categories

Urology and Nephrology

Medicinal Chemistry

Nutrition and Dietetics





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