Saccharomyces cerevisiae strains performing similarly during fermentation of lignocellulosic hydrolysates show pronounced differences in transcriptional stress responses
Journal article, 2024

Improving our understanding of the transcriptional changes of Saccharomyces cerevisiae during fermentation of lignocellulosic hydrolysates is crucial for the creation of more efficient strains to be used in biorefineries. We performed RNA sequencing of a CEN.PK laboratory strain, two industrial strains (KE6-12 and Ethanol Red), and two wild-type isolates of the LBCM collection when cultivated anaerobically in wheat straw hydrolysate. Many of the differently expressed genes identified among the strains have previously been reported to be important for tolerance to lignocellulosic hydrolysates or inhibitors therein. Our study demonstrates that stress responses typically identified during aerobic conditions such as glutathione metabolism, osmotolerance, and detoxification processes also are important for anaerobic processes. Overall, the transcriptomic responses were largely strain dependent, and we focused our study on similarities and differences in the transcriptomes of the LBCM strains. The expression of sugar transporter-encoding genes was higher in LBCM31 compared with LBCM109 that showed high expression of genes involved in iron metabolism and genes promoting the accumulation of sphingolipids, phospholipids, and ergosterol. These results highlight different evolutionary adaptations enabling S. cerevisiae to strive in lignocellulosic hydrolysates and suggest novel gene targets for improving fermentation performance and robustness.

wild-type isolates


inhibitor stress

RNA sequencing

industrial yeast strains


Elena Cámara

Chalmers, Life Sciences, Industrial Biotechnology

Maurizio Mormino

Chalmers, Life Sciences, Industrial Biotechnology

Verena Siewers

Chalmers, Life Sciences, Systems and Synthetic Biology

Yvonne Nygård

Chalmers, Life Sciences, Industrial Biotechnology

Applied and Environmental Microbiology

0099-2240 (ISSN) 1098-5336 (eISSN)

Vol. 90 5

Complete Carbon-LA - Lactic acid from the entire biomass

Formas (2022-01465), 2023-01-01 -- 2026-12-31.

Subject Categories

Biochemistry and Molecular Biology


Bioinformatics and Systems Biology





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