Site-selective immobilization of functionalized DNA origami on nanopatterned Teflon AF
Journal article, 2017
We demonstrate the use of arrays of Teflon AF nanopillars for directing the assembly of single rectangular DNA origami scaffolds, functionalized with covalently linked fluorophore molecules, in defined positions on patterned surfaces. This is achieved by introducing Teflon AF as a non-amplified negative e-beam resist, which is exposed and chemically developed to generate arrays of hydrophobic nanopillars with a minimum feature size 40 nm. Binding of the DNA origami to the pillars is facilitated by porphyrin moieties that act as hydrophobic molecular anchors, reaching 80% coverage of the available sites. This combination of top-down lithography and bottom-up self assembly is an efficient means of fabricating hierarchically structured bio-nanointerfaces in which the positioning of functional units is precisely controlled on the molecular scale inside the DNA assembly, and on the nanoscale at pre-designed locations on the substrate.
Author
Mehrnaz Shaali
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Jakob Woller
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Peter Johansson
Chalmers, Physics, Bionanophotonics
Jonas Hannestad
Chalmers, Physics, Biological Physics
Laura De Battice
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Nesrine Aissaoui
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
T. Brown
University of Oxford
Afaf El-Sagheer
University of Oxford
Suez University
Sergey Kubatkin
Chalmers, Microtechnology and Nanoscience (MC2), Quantum Device Physics
Samuel Lara Avila
Chalmers, Microtechnology and Nanoscience (MC2), Quantum Device Physics
Bo Albinsson
Chalmers, Chemistry and Chemical Engineering
Aldo Jesorka
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Journal of Materials Chemistry C
20507526 (ISSN) 20507534 (eISSN)
Vol. 5 30 7637-7643Areas of Advance
Nanoscience and Nanotechnology
Subject Categories
Physical Chemistry
Other Physics Topics
DOI
10.1039/c7tc01015j