Mechanical characterization of base analogue modified nucleic acids by force spectroscopy
Journal article, 2021
We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study highlights the benefit of using base-modified nucleic acids in force-spectroscopy.
Author
Vinoth Sundar Rajan
Chalmers, Chemistry and Chemical Engineering
Chalmers, Biology and Biological Engineering, Chemical Biology
Xavier Viader-Godoy
University of Barcelona
Yii Lih Lin
Chalmers, Biology and Biological Engineering, Chemical Biology
Uttama Dutta
Chalmers, Biology and Biological Engineering
Chalmers, Chemistry and Chemical Engineering
Fredrik Westerlund
Chalmers, Biology and Biological Engineering, Chemical Biology
Marcus Wilhelmsson
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Physical Chemistry Chemical Physics
1463-9076 (ISSN) 1463-9084 (eISSN)
Vol. 23 26 14151-14155Subject Categories
Analytical Chemistry
Biomedical Laboratory Science/Technology
Biophysics
DOI
10.1039/d1cp01985f
PubMed
34180930