A new approach to measuring vitamin D in human adipose tissue using time-of-flight secondary ion mass spectrometry: A pilot study
Artikel i vetenskaplig tidskrift, 2014

Circulating concentrations of vitamin D, 25(OH)D, and 1,25(OH)2D are lower in obese than lean individuals, but little is known about the adipose tissue content of these molecules. The aim of this study was to explore the possibility to use time-of-flight secondary ion mass spectrometry (TOF-SIMS) to measure vitamin D and its metabolites in fat tissue in obese and lean subjects. Abdominal subcutaneous adipose tissue (SAT) biopsies were obtained from three lean and three obese women, and paired biopsies SAT and visceral adipose tissue (VAT) were obtained from three obese subjects during gastric bypass surgery. TOF-SIMS was used to measure vitamin D3, 25(OH)D3, and 1,25(OH)2D3 in adipose tissue. We found that vitamin D3, 25(OH)D3, and 1,25(OH)2D3 in adipose tissue can be measured with TOF-SIMS. In adipose tissue, vitamin D3 and its metabolites were located in adipocyte lipid droplets. The content of vitamin D3 (P = 0.006) and 25(OH)D3 (P = 0.018) were lower in SAT in obese compared with lean women. TOF-SIMS has the potential to semi-quantitatively measure vitamin D metabolites in adipose tissue, and offers a possibility to compare vitamin D levels in different depots and groups of individuals. It also gives the opportunity to explore the localization of vitamin D metabolites at a cellular level.

Adipose tissue

TOF-SIMS

Vitamin D

Författare

Per Malmberg

Chalmers, Kemi- och bioteknik, Analytisk kemi

Therese Karlsson

Göteborgs universitet

Henrik Svensson

Göteborgs universitet

Malin Lönn

Göteborgs universitet

Nils-Gunnar Carlsson

Kemi- och bioteknik, Livsvetenskaper, Livsmedelsvetenskap

Ann-Sofie Sandberg

Kemi- och bioteknik, Livsvetenskaper, Livsmedelsvetenskap

Eva Jennische

Göteborgs universitet

Amra Osmancevic

Sahlgrenska universitetssjukhuset

Agneta Holmäng

Göteborgs universitet

Journal of Photochemistry and Photobiology B: Biology

1011-1344 (ISSN)

Vol. 138 295-301

Ämneskategorier

Dermatologi och venereologi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1016/j.jphotobiol.2014.06.008

PubMed

25010290