Using nanofluidic channels to probe dynamics of RAD51-Filaments
Paper i proceeding, 2015

Using nanochannels, passivated with a lipid bilayer to avoid sticking of proteins, we study Rad51 filaments bound to single- and double stranded DNA. We demonstrate how we can discern different properties of the filaments by studying them at different degrees of confinement. Unlike the bacterial homologue RecA, that forms homogeneous filaments along DNA, Rad51 forms heterogeneous filaments containing both rigid kinks as well as flexible regions. Varying the counterion, the DNA substrate as well as the initial protein concentration, we try to understand the factors governing the structure of the filaments.

DNA-protein interactions

Lipid passivation




Louise Fornander

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Mohammadreza Alizadehheidari

Chalmers, Kemi och kemiteknik, Kemi och biokemi

E. Werner

Göteborgs universitet

A. Çekir

Göteborgs universitet

Joachim Fritzsche

Chalmers, Teknisk fysik, Kemisk fysik

J. Araya

Northeastern University

P. Nevin

Northeastern University

P. J. Beuning

Northeastern University

Bernhard Mehlig

Göteborgs universitet

M. Modesti

Aix-Marseille Université

Karolin Frykholm

Chalmers, Biologi och bioteknik, Kemisk biologi

F. Persson

Uppsala universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

978-097980647-6 (ISBN)


Nanovetenskap och nanoteknik

Livsvetenskaper och teknik (2010-2018)


Biokemi och molekylärbiologi



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