Using nanofluidic channels to probe dynamics of RAD51-Filaments
Paper i proceeding, 2015

Using nanochannels, passivated with a lipid bilayer to avoid sticking of proteins, we study Rad51 filaments bound to single- and double stranded DNA. We demonstrate how we can discern different properties of the filaments by studying them at different degrees of confinement. Unlike the bacterial homologue RecA, that forms homogeneous filaments along DNA, Rad51 forms heterogeneous filaments containing both rigid kinks as well as flexible regions. Varying the counterion, the DNA substrate as well as the initial protein concentration, we try to understand the factors governing the structure of the filaments.

Lipid passivation

DNA-protein interactions




Louise Fornander

Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Mohammadreza Alizadehheidari

Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

E. Werner

Göteborgs universitet

A. Çekir

Göteborgs universitet

Joachim Fritzsche

Chalmers, Teknisk fysik, Kemisk fysik

J. Araya

Northeastern University

P. Nevin

Northeastern University

P. J. Beuning

Northeastern University

Bernhard Mehlig

Göteborgs universitet

M. Modesti

Aix Marseille Universite

Karolin Frykholm

Chalmers, Biologi och bioteknik, Kemisk biologi

F. Persson

Uppsala universitet

Fredrik Westerlund

Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014



Nanovetenskap och nanoteknik

Livsvetenskaper och teknik


Biokemi och molekylärbiologi