Untargeted 1H NMR-Based Metabolomics Analysis of Urine and Serum Profiles after Consumption of Lentils, Chickpeas, and Beans: An Extended Meal Study to Discover Dietary Biomarkers of Pulses
Artikel i vetenskaplig tidskrift, 2018

High legume intake has been shown to have beneficial effects on the health of humans. The use of nutritional biomarkers, as a complement to self-reported questionnaires, could assist in evaluating dietary intake and downstream effects on human health. The aim of this study was to investigate potential biomarkers of the consumption of pulses (i.e., white beans, chickpeas, and lentils) by using untargeted NMR-based metabolomics. Meals rich in pulses were consumed by a total of 11 participants in a randomized crossover study and multilevel partial least-squares regression was employed for paired comparisons. Metabolomics analysis indicated that trigonelline, 3-methylhistidine, dimethylglycine, trimethylamine, and lysine were potential, though not highly specific, biomarkers of pulse intake. Furthermore, monitoring of these metabolites for a period of 48 h after intake revealed a range of different excretion patterns among pulses. Following the consumption of pulses, a metabolomic profiling revealed that the concentration ratios of trigonelline, choline, lysine, and histidine were similar to those found in urine. In conclusion, this study identified potential urinary biomarkers of exposure to dietary pulses and provided valuable information about the time-response effect of these putative biomarkers.

legumes

NMR

dietary pulses

biomarkers

metabolomics

Författare

Francisco Madrid-Gambin

Universitat de Barcelona

Instituto de Salud Carlos III

Carl Brunius

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Mar Garcia-Aloy

Instituto de Salud Carlos III

Universitat de Barcelona

Sheila Estruel-Amades

Universitat de Barcelona

Rikard Landberg

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Sveriges lantbruksuniversitet (SLU)

Cristina Andres-Lacueva

Universitat de Barcelona

Instituto de Salud Carlos III

Journal of Agricultural and Food Chemistry

0021-8561 (ISSN) 1520-5118 (eISSN)

Vol. 66 27 6997-7005

Optimal kost utifrån metabotyp för hälsa och vällevnad

Formas, 2016-01-01 -- 2021-12-31.

Ämneskategorier

Miljömedicin och yrkesmedicin

Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi

Näringslära

DOI

10.1021/acs.jafc.8b00047

Mer information

Senast uppdaterat

2018-09-19