Interbase FRET in RNA: from A to Z
Artikel i vetenskaplig tidskrift, 2019

Published by Oxford University Press on behalf of Nucleic Acids Research. Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tCO and the non-emissive acceptor tCnitro. The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation.




Anders Foller Füchtbauer

Göteborgs universitet, Institutionen för Kemi

Moa Sandberg Wranne

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Mattias Bood

AstraZeneca AB

Göteborgs universitet

Erik Weis

Göteborgs universitet

AstraZeneca AB

Pauline Pfeiffer

Chalmers, Biologi och bioteknik, Kemisk biologi

Jesper Nilsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Anders Dahlén

AstraZeneca AB

Morten Grötli

Göteborgs universitet

Marcus Wilhelmsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Nucleic Acids Research

0305-1048 (ISSN) 1362-4962 (eISSN)

Vol. 47 19 9990-9997


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