Interbase FRET in RNA: from A to Z
Artikel i vetenskaplig tidskrift, 2019
Published by Oxford University Press on behalf of Nucleic Acids Research. Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tCO and the non-emissive acceptor tCnitro. The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation.
fluorescence
nucleosides
Författare
Anders Foller Füchtbauer
Göteborgs universitet, Institutionen för Kemi
Moa Sandberg Wranne
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Mattias Bood
Göteborgs universitet
AstraZeneca AB
Erik Weis
AstraZeneca AB
Göteborgs universitet
Pauline Pfeiffer
Chalmers, Biologi och bioteknik, Kemisk biologi
Jesper Nilsson
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Anders Dahlén
AstraZeneca AB
Morten Grötli
Göteborgs universitet
Marcus Wilhelmsson
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Nucleic Acids Research
0305-1048 (ISSN) 1362-4962 (eISSN)
Vol. 47 19 9990-9997Styrkeområden
Nanovetenskap och nanoteknik (SO 2010-2017, EI 2018-)
Hälsa och teknik
Ämneskategorier
Fysikalisk kemi
Biofysik
Strukturbiologi
DOI
10.1093/nar/gkz812
PubMed
31544922