Interbase FRET in RNA: from A to Z
Journal article, 2019

Published by Oxford University Press on behalf of Nucleic Acids Research. Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tCO and the non-emissive acceptor tCnitro. The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation.

nucleosides

fluorescence

Author

Anders Foller Füchtbauer

University of Gothenburg, Department of Chemistry

Moa Sandberg Wranne

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Mattias Bood

University of Gothenburg

AstraZeneca AB

Erik Weis

AstraZeneca AB

University of Gothenburg

Pauline Pfeiffer

Chalmers, Biology and Biological Engineering, Chemical Biology

Jesper Nilsson

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Anders Dahlén

AstraZeneca AB

Morten Grötli

University of Gothenburg

Marcus Wilhelmsson

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Nucleic Acids Research

0305-1048 (ISSN) 1362-4962 (eISSN)

Vol. 47 19 9990-9997

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Health Engineering

Subject Categories

Physical Chemistry

Biophysics

Structural Biology

DOI

10.1093/nar/gkz812

PubMed

31544922

More information

Latest update

6/10/2020