Interbase FRET in RNA: from A to Z
Journal article, 2019
Published by Oxford University Press on behalf of Nucleic Acids Research. Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tCO and the non-emissive acceptor tCnitro. The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation.
fluorescence
nucleosides
Author
Anders Foller Füchtbauer
University of Gothenburg, Department of Chemistry
Moa Sandberg Wranne
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Mattias Bood
University of Gothenburg
AstraZeneca AB
Erik Weis
AstraZeneca AB
University of Gothenburg
Pauline Pfeiffer
Chalmers, Biology and Biological Engineering, Chemical Biology
Jesper Nilsson
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Anders Dahlén
AstraZeneca AB
Morten Grötli
University of Gothenburg
Marcus Wilhelmsson
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Nucleic Acids Research
0305-1048 (ISSN) 1362-4962 (eISSN)
Vol. 47 19 9990-9997Areas of Advance
Nanoscience and Nanotechnology (SO 2010-2017, EI 2018-)
Health Engineering
Subject Categories
Physical Chemistry
Biophysics
Structural Biology
DOI
10.1093/nar/gkz812
PubMed
31544922