Specific xylan activity revealed for AA9 Lytic Polysaccharide Monooxygenases of the thermophilic fungus Malbranchea cinnamomea by functional characterization
Artikel i vetenskaplig tidskrift, 2019

The thermophilic biomass-degrader Malbranchea cinnamomea exhibits poor growth on cellulose but excellent growth on hemicelluloses as the sole carbon source. This is surprising considering that its genome encodes eight lytic polysaccharide monooxygenases (LPMOs) from auxiliary activity family 9 (AA9), enzymes known for their high potential in accelerating cellulose depolymerization. We characterized four of the eight (M. cinnamomea AA9s) McAA9s, namely, McAA9A, McAA9B, McAA9F, and McAA9H, to gain a deeper understanding about their roles in the fungus. The characterized McAA9s were active on hemicelluloses, including xylan, glucomannan, and xyloglucan, and furthermore, in accordance with transcriptomics data, differed in substrate specificity. Of the McAA9s, McAA9H is unique, as it preferentially cleaves residual xylan in phosphoric acid-swollen cellulose (PASC). Moreover, when exposed to cellulose-xylan blends, McAA9H shows a preference for xylan and for releasing (oxidized) xylooligosaccharides. The cellulose dependence of the xylan activity suggests that a flat conformation, with rigidity similar to that of cellulose microfibrils, is a prerequisite for productive interaction between xylan and the catalytic surface of the LPMO. McAA9H showed a similar trend on xyloglucan, underpinning the suggestion that LPMO activity on hemicelluloses strongly depends on the polymers’ physicochemical context and conformation. Our results support the notion that LPMO multiplicity in fungal genomes relates to the large variety of copolymeric polysaccharide arrangements occurring in the plant cell wall.

hemicellulose

monooxygenases

biomass degradation

thermophilic

Författare

Silvia Hüttner

Chalmers, Biologi och bioteknik, Industriell bioteknik

Anikó Varnai

Norges miljø- og biovitenskapelige universitet

Dejan Petrović

Norges miljø- og biovitenskapelige universitet

Cao Xuan Bach

Chalmers, Biologi och bioteknik

Kim Anh Dang Thi

Chalmers, Biologi och bioteknik, Industriell bioteknik

Vu Nguyen Thanh

Food Industries Research Institute

Vincent G.H. Eijsink

Norges miljø- og biovitenskapelige universitet

Johan Larsbrink

Chalmers, Biologi och bioteknik, Industriell bioteknik

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

Applied and Environmental Microbiology

0099-2240 (ISSN) 1098-5336 (eISSN)

Vol. 85 23

Enzymer upptäckt och produktion i Vietnam för produktion av hållbara biobränslen och biokemikalier

Vetenskapsrådet (VR), 2014-12-01 -- 2016-12-31.

Drivkrafter

Hållbar utveckling

Ämneskategorier

Industriell bioteknik

Biokemi och molekylärbiologi

Mikrobiologi

Styrkeområden

Energi

Livsvetenskaper och teknik (2010-2018)

Fundament

Grundläggande vetenskaper

DOI

10.1128/AEM.01408-19

PubMed

31540984

Mer information

Senast uppdaterat

2020-01-20