Small scale screening of yeast strains enables high-throughput evaluation of performance in lignocellulose hydrolysates
Artikel i vetenskaplig tidskrift, 2020

Second generation biorefineries demand efficient lignocellulosic hydrolysate fermenting strains and recent advances in strain isolation and engineering have progressed the bottleneck in developing production hosts from generation of strains into testing these under relevant conditions. In this paper, we introduce a methodology for high-throughput analysis of yeast strains directly in lignocellulosic hydrolysates. The Biolector platform was used to assess aerobic and anaerobic growth of 12 Saccharomyces cerevisiae strains and their ΔPdr12 mutants in wheat straw hydrolysate. The strains evaluated included lab, industrial and wild type strains and the screening could capture significant differences in growth and ethanol production among the strains. The methodology was also demonstrated with corn stover hydrolysate and the results were in line with shake flask cultures. Our study demonstrates that growth in lignocellulosic hydrolysates could be rapidly monitored using 1 ml cultures and that measuring growth and product formation under relevant conditions are crucial for evaluating strain performance.

Wheat straw hydrolysate

Strain evaluation

Bioethanol

Saccharomyces cerevisiae

Adaptation

Författare

Marlous van Dijk

Chalmers, Biologi och bioteknik, Industriell bioteknik

Ignatius Trollmann

Chalmers, Biologi och bioteknik

Margarete Saraiva

Chalmers, Biologi och bioteknik, Industriell bioteknik

Rogelio Lopes Brandão

Universidade Federal de Ouro Preto

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

Yvonne Nygård

Chalmers, Biologi och bioteknik, Industriell bioteknik

Bioresource Technology Reports

2589014X (eISSN)

Vol. 11 100532

Xylosfermentering och cellpropagering för effektiv produktion av cellulosabaserad etanol

Energimyndigheten, 2016-01-01 -- 2019-12-31.

Drivkrafter

Hållbar utveckling

Styrkeområden

Energi

Ämneskategorier

Mikrobiologi

Annan industriell bioteknik

DOI

10.1016/j.biteb.2020.100532

Mer information

Senast uppdaterat

2020-12-01