A LCMS Metabolomic Workflow to Investigate Metabolic Patterns in Human Intestinal Cells Exposed to Hydrolyzed Crab Waste Materials
Artikel i vetenskaplig tidskrift, 2021

We have developed a LCMS metabolomic workflow to investigate metabolic patterns from human intestinal cells treated with simulated gastrointestinal-digested hydrolyzed crab waste materials. This workflow facilitates smart and reproducible comparisons of cell cultures exposed to different treatments. In this case the variable was the hydrolysis methods, also accounting for the GI digestion giving an output of direct correlation between cellular metabolic patterns caused by the treatments. In addition, we used the output from this workflow to select treatments for further evaluation of the Caco-2 cell response in terms of tentative anti-inflammatory activity in the hopes to find value in the crab waste materials to be used for food products. As hypothesized, the treatment identified to change the cellular metabolomic pattern most readily, was also found to cause the greatest effect in the cells, although the response was pro-inflammatory rather than anti-inflammatory, it proves that changes in cellular metabolic patterns are useful predictors of bioactivity. We conclude that the developed workflow allows for cost effective, rapid sample preparation as well as accurate and repeatable LCMS analysis and introduces a data pipeline specifically for probe the novel metabolite patterns created as a means to assess the performing treatments.

metabolomic

Caco-2

workflow

LCMS

cells

Författare

Fionn Ofearghail

Technological University Dublin

Patrice Behan

Technological University Dublin

Niklas Engström

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Nathalie Scheers

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Frontiers in Bioengineering and Biotechnology

2296-4185 (ISSN)

Vol. 9 629083

Exploring Shellfish By-Products as sources of BlueBioactivities (BLUESHELL)

Västra Götalandsregionen, 2017-04-01 -- 2020-03-30.

Ämneskategorier

Annan biologi

Farmakologi och toxikologi

DOI

10.3389/fbioe.2021.629083

Mer information

Senast uppdaterat

2021-03-18