Extracts of Digested Berries Increase the Survival of Saccharomyces cerevisiae during H2O2 Induced Oxidative Stress
Artikel i vetenskaplig tidskrift, 2021

Many studies suggest anthocyanins may prevent the development of several diseases. However, anthocyanin bioactivity against cellular stress is not fully understood. This study aimed to evaluate the protective effect of berry anthocyanins on stressed cells using Saccharomyces cerevisiae. The impact of in vitro gastrointestinal digestion on anthocyanin profiles was also assessed. Bilberry and blackcurrant had higher anthocyanin levels than raspberry and strawberry, but digestion reduced the detected anthocyanins by approximately 90%. Yeast cells with and without digested or nondigested anthocyanin extracts were exposed to H2O2 and examined for survival. In the presence of anthocyanins, particularly from digested strawberry, a significant increase in cell survival was observed, suggesting that the type and levels of anthocyanins are important factors, but they also need to undergo gastrointestinal (GI) structural modifications to induce cell defence. Results also showed that cells need to be exposed to anthocyanins before the stress was applied, suggesting induction of a cellular defence system by anthocyanins or their derivatives rather than by a direct antioxidative effect on H2O2. Overall, data showed that exposure of severely stressed yeast cells to digested berry extracts improved cell survival. The findings also showed the importance of considering gastrointestinal digestion when evaluating anthocyanins' biological activity.

berry fruits

polyphenols

improved survival

stress bioassay

yeast

in vitro digestion

oxidative stress

anthocyanins

Författare

Gabriel Oliveira

Natasa Radovanovic

Maria Cecilia do Nascimento Nunes

University of South Florida

Rikard Fristedt

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Marie Alminger

Chalmers, Biologi och bioteknik

Thomas Andlid

Grundutbildning Fysik, kemi och bioteknik samt matematik och tekniskt basår

Molecules

1420-3049 (ISSN)

Vol. 26 4 1057

Ämneskategorier

Immunologi

Cell- och molekylärbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.3390/molecules26041057

PubMed

33670455

Mer information

Senast uppdaterat

2021-03-23