Reconstruction, simulation and analysis of enzyme-constrained metabolic models using GECKO Toolbox 3.0
Artikel i vetenskaplig tidskrift, 2024
Genome-scale metabolic models (GEMs) are computational representations that enable mathematical exploration of metabolic behaviors within cellular and environmental constraints. Despite their wide usage in biotechnology, biomedicine and fundamental studies, there are many phenotypes that GEMs are unable to correctly predict. GECKO is a method to improve the predictive power of a GEM by incorporating enzymatic constraints using kinetic and omics data. GECKO has enabled reconstruction of enzyme-constrained metabolic models (ecModels) for diverse organisms, which show better predictive performance than conventional GEMs. In this protocol, we describe how to use the latest version GECKO 3.0; the procedure has five stages: (1) expansion from a starting metabolic model to an ecModel structure, (2) integration of enzyme turnover numbers into the ecModel structure, (3) model tuning, (4) integration of proteomics data into the ecModel and (5) simulation and analysis of ecModels. GECKO 3.0 incorporates deep learning-predicted enzyme kinetics, paving the way for improved metabolic models for virtually any organism and cell line in the absence of experimental data. The time of running the whole protocol is organism dependent, e.g., ~5 h for yeast.
Författare
Yu Chen
Chalmers, Life sciences, Systembiologi
Shenzhen Institute of Advanced Technology
Johan Gustafsson
Chalmers, Biologi och bioteknik
Albert Tafur Rangel
Novo Nordisk Fonden
Petre Mihail Anton
Chalmers, Life sciences, Systembiologi
Iván Domenzain Del Castillo Cerecer
Chalmers, Life sciences, Systembiologi
Cheewin Kittikunapong
Chalmers, Life sciences, Systembiologi
Feiran Li
Chalmers, Life sciences, Systembiologi
Tsinghua University
Le Yuan
Chalmers, Life sciences, Systembiologi
Jens B Nielsen
Chalmers, Life sciences, Systembiologi
BioInnovation Institute
Eduard Kerkhoven
Chalmers, Life sciences, Systembiologi
Novo Nordisk Fonden
Nature Protocols
1754-2189 (ISSN) 17502799 (eISSN)
Vol. 19 3 629-667Optimera mikrobiell tillverkning av itakonat för hållbar plast och tvättmedel
Formas (2018-00597), 2019-01-01 -- 2021-12-31.
Synthetic microbial consortia-based platform for flavonoids production using synthetic biology (Synbio4Flav)
Europeiska kommissionen (EU) (EC/H2020/814650), 2019-01-01 -- 2023-02-28.
Uppvaknande av kryptiska biosyntetiska genkluster med fet röd jäst
Vetenskapsrådet (VR) (2019-04624), 2019-12-01 -- 2023-11-30.
Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds (CHASSY)
Europeiska kommissionen (EU) (EC/H2020/720824), 2016-12-01 -- 2020-11-30.
Ämneskategorier (SSIF 2011)
Biokemi och molekylärbiologi
Bioinformatik (beräkningsbiologi)
Bioinformatik och systembiologi
DOI
10.1038/s41596-023-00931-7
PubMed
38238583